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Fig. 1 Effect of genetic attenuation of tpcn2 on body axis straightening in embryos at ∼24 hpf. Representative bright-field images showing the body axis straightening morphology in embryos at ∼24–25 hpf. Embryos were (A) untreated (control) or injected with (B) standard control (SC)-MO, (C) p53-MO, or (D) tpcn2-MO-T + p53-MO. In addition, representative bright-field images of a (E) tpcn2 heterozygous (tpcn2+/−) and (F) tpcn2 homozygous (tpcn2−/−) mutant are shown. The body axis straightening angle (indicated by the pink dashed lines) was measured. Ant and Pos are anterior and posterior, respectively. Scale bar, 500 µm. (G) Quantification of the body straightening angle. The bar chart shows the mean ± SEM body straightening angle on to which is superimposed the individual data points. The number (n) of embryos in each condition is shown. Statistical analysis was carried out using the Mann–Whitney U-test and data that were significantly different are represented by *p < .05, **p < .01, and ***p < .001.