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Fig. 6 Knockdown of nkx2.3 increased Fgf signal and fgf8 overexpression mimic the phenotype of nkx2.3 morphants. (A) Expression pattern of fgf3 and fgf8 in nkx2.3 morphants detected by ISH at 36 hpf. i, isthmus; os, optic stalk; pvh, posterior-ventral hypothalamus; ep, endodermal pouches; mhb, midbrain-hindbrain boundary; ah, adenohypophysis. (B) Morphology of embryos with overexpressed fgf8. Scale bars: 200 μm. (C) Alcian blue staining of embryos in which fgf8 was overexpressed. Scale bars: 100 μm. (D) Immunostaining of Col2a proteins in fgf8 transgenic embryos at 4 dpf. Scale bars: 10 μm.
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