Fig. 4.

Fig. 4.

mcf2lb mutants show abnormal organization of the pLLP and NM deposition. (A-B″) Lateral views of stills from time-lapse movies obtained between 30 and 44 hpf of either WT or mcf2lb mutants positive for Tg(-8.0claudinB: lynGFP)^zf106 (Movies 1,2). (C) Mean pLLP length in WT (n=7 pLLPs) and mcf2lb mutants (n=8 pLLPs). (D) Mean number of cells within the pLLP in WT (n=19 pLLPs) and mcf2lb mutants (n=19 pLLPs). (E) Mean velocity of the pLLP in WT (n=7 pLLPs) and mcf2lb mutants (n=6 pLLPs). (F-G″) High-magnification lateral views of still images from movies of the migrating pLLP in WT and mcf2lb mutants (Movies 3,4). pLLPs were imaged for ∼1.5 h starting at ∼30 hpf. Note the abnormal organization of the pLLP in mcf2lb mutants and the beads on a string appearance along the midline instead of distinct clusters of membrane as in WT (Movies 3,4). Asterisks indicate foci. Arrowheads indicate points. (H) Mean number of foci per pLLP in WT (n=15 pLLPs) and mcf2lb mutants (n=18 pLLPs). (I) Mean number of points per pLLP in WT (n=15 pLLPs) and mcf2lb mutants (n=18 pLLPs). (J) Scatter plot of foci numbers per pLLP versus number of points per pLLP in WT (n=15 pLLPs) and mcf2lb mutants (n=18 pLLPs). *P<0.05, **P<0.01 (unpaired two-tailed t-test). n.s., not significant. Error bars are s.d. Scale bars: 20 μm (A,B); 10 μm (F,G).