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Fig. 4 HSF1 activity was regulated by substrate stiffness (A) The illustration of the experiment device for substrate stiffness stimuli to cultured cells. (B) Fluorescent immunostaining of phosphorylated HSF1 at Serine 326 in MDCK cells under substrate stiffness stimuli. Scale bar: 10 μm. (C) The ratio of phosphorylated HSF1 at Serine 326 in nuclei and cytoplasm (N/C) in (B) (n = 9). Data are expressed as mean ± SEM, ∗p < 0.05, ∗∗p < 0.01. (D–I) Fluorescence intensity of phosphorylated HSF1 at Serine 326 and DAPI along the lines in the magnified figures in (B).
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