Positive differentiation of immune response markers as shown by principal component analysis. (A) PCA plot of individuals and their distribution that was significantly affected by the immune response variables as indicated in (B). Confidence ellipses drawn around the levels of the categorical variables for treatment status with a confidence level of 0.95; variables plot showing the highest (red) to lowest (blue) contribution of variables to the PCA plot (A). Cathepsin L and Lysozyme indicate markers measured by ELISA; immune gene expression markers are indicated in lower case; CD8 and CD4 indicated specific T-cell markers for adaptive immune cell phenotypes (CD8α+ and CD4+ cells, respectively); tight junction and mucus biomarkers were indicated by ZO- 2a and Muc2.1, respectively.
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