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Fig. 6 Arnt1 and Arnt2 co-immunoprecipitate with Npas4l. (A) HEK293T cells were transfected with zebrafish Npas4l-V5 and Arnt1-myc. Cells were homogenized (input) and immunoprecipitated (IP) with antibodies against V5 or an immunoglobulin G control (IgG). Samples were run on an immunoblot and probed using myc antibodies (anti-myc) to detect the presence of Arnt1-myc (top) or samples were probed using V5 antibodies (anti-V5) to verify successful pulldown of Npas4l-V5 (bottom). (B) HEK293T cells were transfected with zebrafish Npas4l-V5 and Arnt2-HA. Cells were homogenized and immunoprecipitated with antibodies against HA or an IgG control. Samples were run on an immunoblot and probed using V5 antibodies (anti-V5) to detect the presence of Npas4l-V5 (top) or samples were probed using HA antibodies (anti-HA) to verify successful pulldown of Arnt2-HA (bottom).