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Fig. 6 Ephrin A1b regulates HSC specification by regulating wnt16 expression. (A,B) Expression of runx1 in the DA or wnt16 in the somites of uninjected embryos or embryos injected with efna1b mRNA. (C,D) Expression of runx1 in the DA of wild-type and jag2bsgRNA uninjected embryos or embryos injected with efna1b mRNA (C) or efna1bsgRNA uninjected embryos or embryos injected with wnt16 mRNA (D). Black arrowheads indicate runx1 expression in the DA (A,C,D) and wnt16 expression in the somites (B). Graphs in C and D show the distribution of embryos exhibiting ‘high’, ‘middle’ or ‘low’ runx1 expression phenotypes. *P<0.05; ***P<0.001; n.s., not significant. Scale bars: 100 μm (A,C,D); 200 μm (B).