Figure 4

Figure 4

Genetic inhibition of Mavs/Ikbke signaling pathway impairs the induction of inflammation and ISGs in Tg(ifih1_mut) upon poly I:C stimulation. Tg(ifih1_mut) were crossed with nfkb:eGFP and isg15:eGFP reporter lines, injected with poly I:C and crRNA/Cas9 complexes for mavs or ikbke. Nfkb activity (A, B) and isg15 levels (C, D) were analyzed by fluorescence microscopy and quantified. Each dot represents a larva and the mean ± SEM for each experimental group is also shown. Representative merge images of whole larvae were also shown (A, C). (E) Transcript levels of isg15 in larvae from the cross of nfkb:eGFP and Tg(ifih1_mut) were analyzed by RT-qPCR and the results are shown as the mean ± SEM of pooled larvae. p-values were calculated using one-way ANOVA and Tukey multiple range test. **p ≤ 0.01, ****p ≤ 0.0001.