|
Fig. 1 Identification and analysis of differentially expressed genes (DEGs). (A) The RNA-seq experiment design. (B) Representative images of Tg(igfbp5a:GFP) larvae at the indicated stages grown in the control medium or the induction medium. Shown here and in following figures are lateral views of the yolk sac region. Anterior to the left and dorsal up. Note clusters of newly divided NaR cells induced by the induction medium at 104 h post fertilisation (hpf). (C) Pairwise comparison of gene expression abundance between the control and the induced NaR cells. The up- and down-regulated genes were shown in green and red, respectively. Genes that were not changed are shown in blue. (D) Hierarchical clustering of the DEGs in the four control (C1–C4) and four induction groups (I1–I4) of NaR cells. Red colour indicates up-regulated genes and blue colour down-regulated genes. (E) qRT-PCR confirmation of RNA-seq data. Changes (log2) in the mRNA levels of the indicated genes measured by RNA-seq were plotted against those detected by qPCR. The line indicates the linear correlation between the results of RNA-seq and qPCR. (F–H) Gene ontology (GO) analysis results of all (F), up-regulated (G) and down-regulated (H) DEGs. The dot size indicates the number of genes, and the x-axis indicates the identified genes ratio in the relevant GO term. (I, J) Kyoto encyclopedia of genes and genomes (KEGG) analysis of all (I), and up-regulated (J). The dot size indicates the number of identified genes, and the x-axis indicates the genes ratio in the relevant KEGG pathway.