ZFIN Image: Bravo et al., 2023, Fig. 4.

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Figure Caption

Fig. 4. irf8 and csf1a are coexpressed in a subpopulation of pre-follicle cells in the early ovary.

(A) Analysis of expression profiles of indicated genes in specified clusters of ovarian cells represented by dot plot graph. (B to D) UMAP plot of indicated gene’s expression in the 40-dpf ovary in (B) and (C) follicle cells, and (D) reclustered subpopulation of pre-follicle cells. Subpopulation of pre-F cells boxed in (B) and (C). UMAP of reclustered pre-F cells; legend boxed in (D). (E) Gene expression correlation values of indicated genes in specified clusters of ovarian cells represented by Spearman’s Rho correlation analysis. (F to H) Double HCR RNA FISH confocal images of 40-dpf wild-type ovary. lhx9, follicle cells (magenta); csf1a, Csf1rs’ ligand (yellow), and DAPI, DNA (cyan). Regions boxed in (F) show magnified views of (G) lhx9/csf1a-coexpressing MAFCs and (H) csf1a-expressing somatic cells. Scale bars, 10 μm. F, follicle cells; Supp, supporting cells.

Acknowledgments

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