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Fig. 4 The mechanism of liver regeneration after ronidazole treatment is similar to that of Mtz. (A) qPCR analysis of hepatoblast developmental markers (hhex, foxa3, sox9b) and hepatocyte markers (cp, gc) expression after Mtz and ronidazole treatment from BT to Reg 72 h. Values represent mean ± SD, n = 3 biology replicates. Data were analyzed by t-test. *p < 0.05, NS, no significant difference. (B) WISH images showing the expression of hhex during the liver regeneration after Mtz and ronidazole treatment from Reg 0 h to Reg 72 h. (C) WISH images showing the expression of gc during the liver regeneration after Mtz and ronidazole treatment from BT to Reg 72 h. Numbers indicate the proportion of larvae exhibiting the phenotype are shown. qPCR, quantitative real-time PCR; WISH, whole-mount in situ hybridization.