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Fig. 5 Mutation of cald1a and cald1b genes is not lethal for zebrafish larvae Analyses of zebrafish allele frequencies and genotype of offspring. Fish heterozygous for both cald1a and cald1b mutation were incrossed in a dihybrid cross. All embryos were genotyped and observed counts (n = 217) were statistically compared to expected counts using binomial (A and B) or Chi-square (C, D, and E) test. (A) Count of detected alleles of cald1a wild-type (marked with A) and sa24667 mutant (a) allele. (B) Count of detected alleles of cald1b wild-type (marked with B) and sa16974 mutant (b) allele. (C) Distribution of cald1a genotypes. (D) Distribution of cald1b genotypes. (E) Distribution of cald1a, cald1b dihybrid genotypes.