ZFIN Image: Hau et al., 2023, Fig. S1

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Figure Caption

Fig. S1 Nonsense-mediated decay of smyhc1 mRNA and lack of compensatory upregulation by smyhc2 or smyhc3 mRNAs. (A) Schematic showing the design of RT-qPCR primers for smyhc1-3 transcripts. To ensure specificity, primer sets were designed to target the 5′ or 3′ untranslated region (UTR) of the transcripts. (B) Expression of smyhc1-3 at 1, 2 and 5 dpf in siblings from smyhc1^kg179/+;Tg(smyhc1:EGFP)^i104/+ in-crosses that had been sorted at 1 dpf into motile and immotile groups. Total RNA prepared from 5 or 10 embryos/larvae per sample from two to four independent lays (represented by symbol shape). Some embryos/larvae were further sorted based on EGFP fluorescence (represented by symbol colour). Bars show mean ± SEM of all samples. Statistics are two-way ANOVA (Stage/Motility) with Bonferroni's post hoc test.

Acknowledgments

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Reprinted from Developmental Biology, 499, Hau, H.A., Kelu, J.J., Ochala, J., Hughes, S.M., Slow myosin heavy chain 1 is required for slow myofibril and muscle fibre growth but not for myofibril initiation, 47-58, Copyright (2023) with permission from Elsevier. Full text @ Dev. Biol.