Fig. 5

Fig. 5

a Electrical stimulation of the hippocampal Schaffer collateral pathway was used to elicit GreenT-EC responses monitored by two-photon microscopy. b Representative example of GreenT-EC expressing hippocampal organotypic slice (left) and the fluorescence traces upon electrical stimulation (right). White ROIs shown in the image correspond to individual traces. The dark-green trace (Full) correspond to a ROI covering the whole image. Scale bar 10 μm. c GreenT-EC fluorescence responses (top) upon spontaneous neuronal activity recorded extracellularly (bottom). d Activity evoked hippocampal CA1 GreenT-EC signals and their inhibition by a combination of sodium ortho-vanadate (SOV, 5 µM) and benzamyl hydrochlorate hydrate (BHH, 50 µM) at 30 min after drug infusion (n = 6, p = 0.0013). e–g Activity evoked hippocampal CA1 GreenT-EC signals and their inhibition after 30 min application of TTX (n = 6, p = 0.0362), CdCl₂ (n = 6, p = 0.0038) or CNQX (n = 6, p = 0.3976). In all cases, n correspond to biological replicates and a parametric two-tailed paired t-test was used to evaluate the differences among control and treated groups (* p < 0.05, ** p < 0.01). Source data are provided in the Source Data file.