Fig. 4

Fig. 4

Proliferation and differentiation markers in hrh1^−/− zebrafish. a Representative pictures (ventral view) for immunohistochemistry of HuC/D in hrh1^+/+ and hrh1^−/− larval brain at 6 dpf. Anterior to the left. Scale bar: 100 µm. N = 6. c Representative pictures (dorsal view) for immunohistochemistry of PCNA in hrh1^+/+ and hrh1^−/− larval brain at 6 dpf. Anterior to the left. Scale bar: 100 µm. N = 6. i Representative pictures (ventral view) for in situ hybridization of pax2a and pax6a in hrh1^+/+ and hrh1^−/− larval brain at 6 dpf. Anterior to the left. Scale bar: 100 µm. b, d–h qPCR results of elavl3, pcna, olig2, slc1a3a, gfap, and isl1 in whole larval zebrafish at 6 dpf. N (hrh1^+/+) = 5, N (hrh1^−/.⁻) = 6. All histograms are exhibit as mean ± SEM, unpaired t test, two tailed. **0.001 < P ≤ 0.01