Fig. 3

Fig. 3

Neurogenesis markers in hrh1^−/− zebrafish. a Representative pictures for in situ hybridization of nes in hrh1^+/+ and hrh1^−/− larval brain at 6 dpf in both dorsal and lateral view. Scale bar: 100 µm. Anterior to the left. N (hrh1^+/+) = 5, N (hrh1^−/−) = 6. c Representative pictures (dorsal view) for in situ hybridization of notch1a in hrh1^+/+ and hrh1^−/− larval brain at 6 dpf. Anterior to the left. Scale bar: 100 µm. N (hrh1^+/+) = 8, N (hrh1^−/−) = 8. b, d, e qPCR results of nes, notch1a, sox2 in whole larval zebrafish at 6 dpf. N (hrh1^+/+) = 5, N (hrh1^−/.⁻) = 6. All bar graphs show mean ± SEM, unpaired t-test, two-tailed. **0.001 < P ≤ 0.01