ZFIN Image: Leino et al., 2023, Fig. 5.

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Figure Caption

Fig. 5.

Expression of Fgf ligands in Zbtb16 mutants. (A,B) HCR for fgf20a in 24 hpf sibling control (n=26) and zbtb16a^−/−;zbtb16b^−/− (n=12) embryos. 3D reconstructions of the z-stack, dorsal view. (C,D) Colorimetric ISH for fgf20b in 24 hpf sibling control (n=15) and zbtb16a^−/−;zbtb16b^−/− (n=4) embryos. Arrows in A-D indicate fgf20-expressing neuronal clusters in rhombomere centres. (E-T) ISH time-course of fgf3 expression in the sibling control (14 hpf: n=19; 16 hpf: n=26; 18 hpf: n=53; 24 hpf: n=47; 30 hpf: n=10; 36 hpf: n=35; 44hpf: n=20; 48 hpf: n=43) and zbtb16a^−/−;zbtb16b^−/− (14 hpf: n=6; 16 hpf: n=10; 18 hpf: n=20; 24 hpf: n=12; 30 hpf: n=5; 36 hpf: n=13; 44 hpf: n=10; 48 hpf: n=12) hindbrain. Arrows in H indicate ectopic expression in r5/r6, arrow in L indicates ectopic expression in the posterior otic vesicle. MHB, midbrain-hindbrain boundary; r, rhombomere. Scale bars: 50 µm.

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