ZFIN Image: Leino et al., 2023, Fig. 3.

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Figure Caption

Fig. 3.

Spatial pattern of neurogenesis in Zbtb16 mutants. (A-F) Colorimetric ISH for neurod4 in 30 hpf sibling control (n=121) and zbtb16a^−/− (n=7) embryos (A,B); 30 hpf sibling control (n=18) and zbtb16a^−/−;zbtb16b^−/− (n=8) embryos (C,D); 44 hpf sibling control (n=8) and zbtb16a^−/−;zbtb16b^−/− (n=8) embryos (E,F). (G-L,O-T) HCR for etv5b, neurod4 and neurog1 in 30 hpf sibling control (n=16) and zbtb16a^−/−;zbtb16b^−/− (n=11) embryos (G-L); 44 hpf sibling control (n=9) and zbtb16a^−/−;zbtb16b^−/− (n=12) embryos (O-T). Sum projections through confocal z-stack. (M,N,U,V) Profile plots of etv5b, neurod4 and neurog1 HCR signal across the hindbrain of representative sibling control (zbtb16a^+/+;zbtb16b^−/−) and zbtb16a^−/−;zbtb16b^−/− embryos; anterior to the left. Scale bars: 50 µm.

Acknowledgments

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