ZFIN Image: Leino et al., 2023, Fig. 2.

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Figure Caption

Fig. 2.

Expression of Zbtb16 during neurogenesis. (A-L) Immunofluorescence for Zbtb16 and HuC/D at 24 and 44 hpf. Dorsal views (A-C,G-I) and transverse sections (D-F,J-L) of the hindbrain. G-I are coronal slices at the level of the mantle zone. Arrows indicate postmitotic neurons (A,B), neurogenic zones (G,H) or Zbtb16+HuC/D double-positive cells (J,L). (M-P) Immunofluorescence for Zbtb16, HuC/D and GFAP at 44 hpf. Side views of the hindbrain, anterior to the left. Arrows indicate Zbtb16-positive migrating progenitors (M) or GFAP-positive glial fibres (O). (Q-V) Two-colour fluorescent ISH for neurog1 and neurod4 (Q-S) or zbtb16a and neurod4 (T-V) at 44 hpf. Side views of the hindbrain, anterior to the left. Arrows indicate overlap of zbtb16a and neurod4 expression. All images are slices from confocal z-stacks. mz, mantle zone; ov, otic vesicle; vz, ventricular zone. Scale bars: 50 µm.

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