Fig. 1

Fig. 1

Fig. 1. SCARB1 deficiency in zebrafish is linked to reduced fertility. (A) Percentage of zebrafish that are WT, heterozygous (HZ) or knock-out (KO)(of total laid eggs) after crossing scarb1^+/− fish (n = 5 individual scarb1^+/− crossings). (B) Representative pictures of adult females and their tails (scarb1 715delA and scarb1 715_716insGG homozygous and sibling controls) at 3 months post-fertilization. (C) Total fertilized egg count at 0 h post fertilization (hpf) produced by WT or scarb1^−/− breeding pairs (n_WT = 15 breeding pairs, n_-1nt = 16 breeding pairs, n_+2nt = 9 breeding pairs). (D) Percentage of survival from 0 hpf till 3 days post fertilization (dpf) of WT and scarb1^−/− zebrafish larvae. (E) Representative picture of the development of scarb1^−/− embryo's compared to WT embryo's at 0 till 3 dpf. (F) Total fertilized egg count at 0 h post fertilization (hpf) produced by outcrosses (♀ WT × ♂ WT (n = 15 breeding pairs), ♀ WT × ♂ scarb1^−/− (n = 5 breeding pairs), ♀ scarb1^−/− × ♂ WT (n = 8 breeding pairs), ♀ scarb1^−/− × ♂ scarb1^−/− (n = 25 breeding pairs)). Means ± SEM; p < 0.05*, p < 0.001*** (One-way ANOVA (multiple groups) or Student's t-test).