Figure 8—figure supplement 1.

Figure 8—figure supplement 1. Hypodermis supports pigment cells.

(A) Iridophores and melanophores in wild-type and bnc2 mutant skins highlighting the relative dearth of pigment cells in the mutant. (A’) Super-resolution images of boxed region in (A), iridophores expressing pnp4a:palm-mCherry and orthogonal projection showing both iridophores and dermal cells expressing ET37:EGFP. bnc2 mutant dermis is bounded by dermal mesenchyme (dm) and lacks the thin, confluent hypodermis seen in wild-type individuals (arrows). (B, C) UMAP visualizations of pigment cell trophic factors, edn3b and csf1b. Compare to cell types illustrated in Figure 1C. (D) bnc2-dependent loss of hypodermis is associated with a greater number of differentially expressed genes (DEGs) in iridophores than in other pigment cell types. Scale bars, 100 μm (A), 10 μm (A’).