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Figure 1
Grouping of experimental animals. Fish were divided into control, vehicle, and MPTP groups. The MPTP1 group received one injection and was further divided based on assessment day, namely 24 h post-injection (MPTP1.1), 72 h post-injection (MPTP1.3), day 5 assessment (MPTP1.5), day 10 assessment (MPTP1.10) and day 30 assessment (MPTP1.30). MPTP2 group received the second dose 24 h after the first dose and was assessed either at 24 h post-second injection (MPTP2.1) or 72 h post-second injection (MPTP2.3). Assessment on all intervals includes a neuro-behavioral assessment from locomotor activity, gene expression, protein level, and histochemical analysis.