ZFIN Image: Vaparanta et al., 2023, Fig. 6

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Fig. 6

Figure 6. NRG-1/ERBB4/STAT5b signaling pathway is perturbed in pathological cardiac hypertrophy

A. Principal component and cluster analysis of transcripts of the NRG-1/ERBB4/STAT5b pathway (including the genes: NRG1, ERBB4, STAT5B, IGF1, MYC and DNM2) with clinical samples representing normal myocardium or hypertrophic cardiomyopathy. The dataset GSE36961 was acquired from the Gene Expression Omnibus database (Data ref: Hebl et al, 2012). One symbol in the plots corresponds to one subject (normal: n = 39, hypertophic cardiomyopathy n = 106; biological replicates). Statistical significance of clusterization was calculated by estimating a probability distribution for the relative distance within a cluster and between clusters and by drawing the cumulative probability from the resulting empirical cumulative probability function.
B. Principal component and cluster analysis of transcripts of the NRG-1/ERBB4/STAT5b pathway with samples representing myocardia of mice subjected to sham or transverse aortic constriction surgery. The dataset GSE5500 was acquired from the Gene Expression Omnibus database (Data ref: Bisping et al, 2006). One symbol in the scatterplots corresponds to one mouse (sham: n = 9, transverse aortic constriction: n = 12; biological replicates).
C, D. Immunohistochemical analysis (C) and quantification (D) of STAT5 activation in sections representing normal myocardia or pathological cardiac hypertrophy (n = 3, aortic stenosis; n = 2, idiopathic cardiomyopathy). Phospho-STAT5b staining intensity was quantified. One dot in the boxplot corresponds to one subject (normal: n = 6, hypertrophic n = 13; biological replicates). In the boxplot the central band represents the median, the box the interquartile range and whiskers the whole range of values. Unpaired two-tailed T-test was used for statistics. Scale bar 50 μm.

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Source Data for Figure 6 [embr202256689-sup-0020-SDataFig6.zip]

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