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Fig. 6
Expression of lgals3bpb among adult tissues. (A) Tissue distribution by western blot, using the 4C4 antibody and βactin as a loading control. Br, brain; E, eyes; Liv, liver, G, gut; H, heart; SkM, skeletal muscle; WKM, whole kidney marrow (n = 3 fish/tissue). kDa, kilodaltons. (B-D) Immunofluorescence on liver (C,D) and gut (E) sections (14 μm) from an adult Tg(mpeg1:GFP) fish. Anti-GFP (green), 4C4 (yellow), anti-Lcp1 (magenta), and a merge including DAPI staining of the three channels are shown. White arrowheads point to GFP+ 4C4+ Lcp1+ cells while empty arrowheads point to GFP− 4C4+ Lcp1+ cells. White arrow in (C) and (D) merged channels show erythrocyte nuclei indicating the presence of a vessel. Images were taken using a 20× objective and correspond to orthogonal projections. (n = 2). Scale bars 50 μm
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