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Fig. 5 Jadomycin B did not cause significant toxicity in female Balb/C mice with 4T1 tumors. Adult female Balb/C mice were injected with 100 000 4T1 cells into the fourth mammary fat pad on day 0, followed by jadomycin B (Jad B) treatment (12 mg/kg every 12 h) from day 6 to 15 after tumor cell implantation. Beginning on day 6, mouse weights were measured every 2 days (A). At the study endpoint, serum was collected and analyzed for alanine amino transferase (B) and creatinine (C) levels as markers of hepatic and renal toxicity, respectively. The data are shown as mean ± SEM (n = 8). ***P ≤ 0.001, compared to day 6, as determined by a two-way repeated measures ANOVA, with Bonferroni multiple comparison text (A). There were no significant differences in serum ALT (B) or creatinine (C) between groups, unpaired t-test.