Image
|
Figure Caption
Fig. 2
IRF. To measure the combined IRF of the excitation laser (Omicron QuixX 375-70PS) and the SPAD camera (Horiba FLIMera), a fraction of the excitation laser pulses (50 MHz pulse repetition rate) was directly imaged onto the FLIMera camera by imaging a retroreflector target in an epi-illuminated widefield microscope setup. The 20-ns time axis consists of 486 time bins of 41.1 ps width. The IRF has an FWHM of 380 ps. The DCR of individual pixels can be measured from the non-zero tail of the pixels’ IRF.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
Full text @ J. Biomed. Opt.