Fig. 5

Fig. 5

Figure 5. Impact of nup133 or elys mutation and nup133 overexpression on NPC assembly

(A–C) Representative immunofluorescence images of NPC signals in nuclei detected by specific antibodies or WGA at 1kc stage. TGnup133, Tg(ef1α:nup133-GFP). The fraction of nuclei with representative signal pattern was indicated.

(D) Average nuclear area in embryos of different backgrounds at 1kc stage. Ne, embryo number. Significant differences were considered as follows: ns, nonsignificance, p ≥ 0.05; ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001.

(E–H) STED microscopic images of individual NPCs labeled with mAb414 or Nup153 at 1kc stage.

(I) Nup153-labeled NPC parameters based on STED images at 1kc stage in (G) and (H). Significant differences were considered as follows: ns, nonsignificance, p ≥ 0.05; ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001.

(J and K) TEM-detected NPCs in nuclei of embryos at 1kc. Representative images of NPCs (indicated by red arrowheads) were shown in (J). NE, nuclear envelope; N, nucleus; C, cytoplasm; Nn, nuclei number; Nnpc, NPC number. (K) Significant differences were considered as follows: ns, nonsignificance, p ≥ 0.05; ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001.

(L–N) Representative FESEM images of isolated nuclear surface embedded with NPCs (L), ratio of NPC types (M), and NPC density (N) in different types of embryos at 1kc stage. Each dot in (N) represented one measurement in a region of interest (ROI). (N) Significant differences were considered as follows: ns, nonsignificance, p ≥ 0.05; ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001.

See also Figure S4.