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Fig. 4
Figure 4. Progressive structural maturity of NPCs during early embryonic development (A) Representative NPCs in embryos observed by TEM. NE, nuclear envelope; Nuc, nucleus; Cyt, cytoplasm. The measurements in (B)–(E) were based on TEM images. (B) Change of NPC density. Significant differences were considered as follows: ns, nonsignificance, p ≥ 0.05; ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001. (C) Change of NPC diameter. Top, illustration of diameter measurement. ONM, outer nuclear membrane; INM, inner nuclear membrane; Nn, nuclei number; Nnpc, NPC number. Significant differences were considered as follows: ns, nonsignificance, p ≥ 0.05; ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001. (D and E) Changes of the relative electron intensity of NPC central pore (D) and the thickness of NE (E). Significant differences were considered as follows: ns, nonsignificance, p ≥ 0.05; ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001. (F) Representative FESEM images of nuclear surface with NPCs embedded. (G) Montages of representative types of NPCs obtained by FESEM. NPCs were masked by transparent cyan. (H) The ratios of different types of NPCs at different developmental stages. Different types were indicated by different colors as shown in (G). (I) Illustration of diameters of FESEM-observed NPCs. (J) Change of NPC diameters at different stages. Only thin rings and mature NPCs were calculated. Significant differences were considered as follows: ns, nonsignificance, p ≥ 0.05; ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001. (K) Illustration of NPCs states during early embryogenesis according to data in (J).
Reprinted from Cell, 185(26), Shen, W., Gong, B., Xing, C., Zhang, L., Sun, J., Chen, Y., Yang, C., Yan, L., Chen, L., Yao, L., Li, G., Deng, H., Wu, X., Meng, A., Comprehensive maturity of nuclear pore complexes regulates zygotic genome activation, 4954-4970.e20, Copyright (2022) with permission from Elsevier. Full text @ Cell