Fig. 4

Fig. 4 Characterization of nphp8^sa10096 and nphp8^sa24730 mutant zebrafish lines. (A) Homozygote nphp8^sa10096 (m/m) zebrafish displayed an increased frequency of cloaca malformation and glomerular cysts. (B) The homozygotic (m/m) incross (F2 generation) showed an increase in glomerular cyst and cloaca malformation. (C) The combined number of glomerular and cloaca cyst formation declined significantly from the nphp8^sa10096 (m/m) F1 to the F2 generation. (D) Heterozygote nphp8^sa24730 (+/m) and homozygote nphp8^sa24730 (m/m) zebrafish displayed an increased frequency of cloaca malformation and glomerular cysts. (E) While glomerular cyst formation was not more frequent between wild-type siblings and the mutant F2 generation (m/m), cloaca malformation increased significantly. (F) Comparison between the F1 and F2 generation, combining glomerular cyst and cloaca malformation, revealed a decline of combined glomerular cyst and cloaca malformation similar to the nphp8^sa10096 mutation. The number of examined embryos is depicted below the graphs. All P values were calculated, using the two-sided Fisher’s exact test.