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Fig. 6
Figure 6. BMP inhibitor exposure followed by two probiotics treatments in Tg(col10a1a:col10a1a-GFP) larvae. (a) Integrated pixel intensity values for various areas measured in 10 dpf zebrafish Tg(col10a1a:col10a1a-GFP) larvae divided into various groups—control, DMSO, LDN, LDN+BS and LDN+LL—and stained with AR. DMSO was used as additional control since it was employed as the solvent for LDN. One-way ANOVA and Tukey’s multiple comparison tests were used, and statistical significance was set at p < 0.05. Different letters denote statistically significant differences among the experimental groups. (b) GFP, AR staining and merged images of the head area (lateral and ventral views) of Tg(col10a1a:col10a1a-GFP) larvae of the different treated groups. White arrows denote GFP in various bony structures and the white arrowhead indicates the presence of a signal in additional structures (that was absent in other groups) in BS-treated fish after LDN exposure (LDN + BS).
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