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Figure 1
Zebrafish embryo dechorionation steps. (A) Using forceps to remove the chorions of 24 hpf embryos: (B) 24 hpf embryos transferred into a 60 mm × 15 mm dish prior to pronase treatment; (C) 24 hpf embryos immediately after 14 min treatment with 0.5 mg/mL pronase and 3X rinse with ICS water; (D) dechorionated 24 hpf embryos in a 12-well plate prior to treatment with TSA or control DMSO.
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