ZFIN Image: Snodgrass et al., 2023, Fig. 4.

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Figure Caption

Fig. 4.

MEK or TOR inhibition normalises the vascular phenotype of eng^mu130 embryos. WT and eng^mu130 mutant embryos were treated with the MEK inhibitor PD0325901 (10 µM) or with the TOR inhibitor rapamycin (2.5 µM) for 24 h between 2 and 3 dpf, or with DMSO vehicle. (A) Representative maximum-intensity projections of the trunk vessels of eng^mu130 and WT embryos±PD0325901 (10 µM) or rapamycin (2.5 µM) treatment for 24 h. Scale bar: 150 μm. (B) Representative maximum-intensity projections of the cerebral vessels of eng^mu130 and WT embryos±PD0325901 (10 µM) or rapamycin (2.5 µM) treatment for 24 h. Scale bar: 100 μm. (C,D) eng^mu130 mutant embryos show reduced DA (C) and PCV (D) diameter following PD0325901 treatment, with PCV diameter normalised to WT values. (E,F) eng^mu130 mutant embryos show reduced DA diameter (E) and PCV diameter (F) following rapamycin treatment, with PCV diameter normalised to WT values. (G) eng^mu130 mutant embryos show normalised kugel formation following PD0325901 treatment. (H) eng^mu130 mutant embryos show normalised kugel formation following rapamycin treatment. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001; ns, not significant (two-way ANOVA with Tukey post-hoc test, 10-14 animals/group).

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Acknowledgments

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