ZFIN Image: Falat et al., 2022, Fig. 6

Image

Figure Caption

Fig. 6 Laminin-dependent anisotropic cell shape hierarchy extends posteriorly to the hindbrain. (A–H) Representative digital slice images of cells in the anterior-posterior (x-axis) and dorsal-ventral (z-axis) dimensions of embryos injected with memGFP and imaged at 24 ss. Cells 40 μm posterior to the MHBC toward the hindbrain are outlined in yellow within the apical region (A–D) and basal edge (E–H). (I–J) Apical and basal quantification of posterior cell anisotropic cell shape (x:z ratio). Boxplots indicate the 25th and 75th percentiles and the median. At least three independent experiments are represented. n = 9 embryos for WT, 8 embryos for lama1 mutants, 6 embryos for lamb1 mutants, and 6 for lamc1 mutants. Significance values ∗∗∗P < 0.005. Scale bars 20 μm.

Acknowledgments

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Reprinted from Developmental Biology, 492, Falat, E.J., Voit, G.C., Gutzman, J.H., Laminin-111 mutant studies reveal a hierarchy within laminin-111 genes in their requirement for basal epithelial tissue folding, 172-186, Copyright (2022) with permission from Elsevier. Full text @ Dev. Biol.