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Figure 3
RNA sequencing and validation of the top genes by RT-qPCR in NAFLD zebrafish supplemented with VitD. (A) Heat map of top differentially expressed protein-coding genes. The expression of 12 genes was downregulated significantly in high VitD-supplemented as compared to NAFLD group (FDR-adjusted p < 0.05). (B) Gene expression by RT-qPCR in NAFLD, NAFLD plus high VitD, and caloric restriction groups. * p < 0.05.
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