Figure 1

EGFP and CYP3A7 expression in hCYP3A7/EGFP-expressing zebrafish. A pT2A plasmid containing hCYP3A7/EGFP (3A7: C,D,G,H,J–L) was injected together with transposase cmRNA into 1-cell stage embryos. (A–D): 17 hpf, (E–H): 50 hpf. EGFP fluorescence in wild-type (WT) zebrafish (B,F) and that in hCYP3A7-expressing zebrafish (hCYP3A7 zebrafish, 3A7) (C,D,H) are shown. Image (A) is a bright field image of a wild-type embryo. Images (E,G) are bright field images of (F,H), respectively. CYP3A7 mRNA expression was observed at 23 hpf by WISH (I–L). Arrowheads indicate examples of positive signals in J–L. Scale bars = 200 µm.