ZFIN Image: Sobah et al., 2023, Figure 4

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Figure 4

Effect of socs3b ablation on neutrophil maturation and functionality. Wildtype (WT, socs3b^wt/wt) and knockout (KO, socs3b^mdu24/mdu24) embryos on the Tg(mpx::GFP) transgenic background at 5 dpf imaged by light (A, B) and fluorescence (C, D) microscopy or subjected to Sudan black staining (E, F), showing representative images in each case. Embryos were also subjected to microinjection with LPS at 5 dpf and imaged by fluorescence microscopy 8 hours later (G–J), showing representative images. Scale bars represent 100 μm. Quantitation of Sudan black⁺(K) or mpx⁺ cells (L) with mean and SEM shown, with significance tested using one-way ANOVA (***p < 0.001, *p < 0.05, n = 8-12).

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Acknowledgments

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