FIGURE 2

Shortening of the Eye Axial Length Induces a Hyperopic Shift in Adult Gjd2b/Cx35.1^−/− Fish. Representative SD-OCT (A) B-mode and (B) en face images of a zebrafish eye (scale bars = 500 µm); geometrical parameters extracted from the OCT datasets are shown and annotated with different colors; the spherical lens is seen as an oval in the B-mode image (panel A) due to a significantly higher refractive index of lens compared to soft tissue. The quantification of (C) the body length (mm), (D) the lens ratio (µm/µm) as a measure of circulatiry, and (E) the retinal thickness (µm) revealed no statistically significant difference between TL controls and gjd2b ^−/− /Cx35.1^−/−. However, the (F) axial length (from top of the lens to the RPE) normalized by body length (µm/mm), the (G) lens radius (µm), and the (H) retinal radius (from the center of the lens to the RPE; µm) collectively showed significantly smaller values (i.e., reduction in size of the eye) in gjd2b ^−/− /Cx35.1^−/−. The (I) calculated relative refractive error (RRE; see text for definition) was significantly higher in gjd2b ^−/− /Cx35.1^−/− which suggested a hyperopic shift, presumably, due to loss of gjd2b/Cx35.1 function. The sample size (N) was 26 for each of the TL and gjd2b ^−/− /Cx35.1^−/− adult fish groups.