ZFIN Image: Sheppard et al., 2023, FIGURE 3

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Figure Caption

FIGURE 3

Zebrafish embryo-based assay for SMAD3 variant pathogenesis. (A) Degree of conservation in amino acid sequence between Smad3a in zebrafish and human SMAD3. Overall, similarity is at ∼97% indicating a very high level of conservation between the species. Residue V244 is highlighted to emphasize total conservation between zebrafish and human Smad3. (B) Confocal images of the tail aorta/vein labeled in Tg[kdrl:mCherry] in 48hpf embryos. White dashed boxes indicate the enlargement regions used to highlight differences in diameter (white dashed lines). Embryos imaged were either control, injected with smad3a^WT mRNA or smad3a^T261I or smad3a^V244F. (C) Quantification of diameter counts. Each point represents an individual embryo measured. Standard deviation is depicted in red. *p < 0.05, ***p < 0.001.

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Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cardiovasc Med