Fig. 4

RREB1 knockout reduces cellular insulin content in mature beta cells. (a–c) RREB1 knockout (RREB1-KO) EndoC-βH1 cells were assessed for (a) INS gene expression (% of control cells [EV], normalised to housekeeping genes TBP and PPIA), (b) cellular insulin content (pg/cell) and (c) glucose-stimulated insulin secretion (pg/ml) (n=5). (d) Forskolin-mediated insulin secretion at 20 mmol/l glucose (pg/ml) (n=3). (e–g) After stimulation with forskolin, (e) glucose-stimulated insulin secretion (pg/ml), (f) the stimulation index and (g) insulin content (pg/cell) were measured (n=3). Data are presented as means±SEM. *p<0.05, **p<0.01, p***<0.001 (a, b, d, unpaired t test; c, e, two-way ANOVA followed by Sidak’s multiple comparisons test)