ZFIN Image: Smits et al., 2022, Fig. 2

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Figure Caption

Fig. 2

Subcellular localization of CLEC16A-WT and -Δ19. a Immunocytochemistry assessing co-localization of transiently expressed pEGFP-CLEC16A-WT (green) with the late endosomal/lysosomal marker LAMP2 (red). Nuclei were counterstained with DAPI. b Partial zoom of a, showing 3.5 × enlargement. c, d Immunocytochemistry assessing the localization of transiently expressed pEGFP-CLEC16A-WT (green, upper panel) and pEGFP-CLEC16A-Δ19 (lower panel) in HEK293T cells. Cells were stained for the early endosomal marker EEA1 (red). Nuclei were counterstained with DAPI. Overview images were made with confocal microscopy (c) and high-resolution images were with 3D-SIM super-resolution microscopy (d). Two representative images of pEGFP-CLEC16A-WT made with 3D-SIM super-resolution microscopy are shown in d. Scale bars represent 5 µm (a, c) and 1 µm (d)

Acknowledgments

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