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Figure 3

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ZDB-IMAGE-230228-311
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Figures for Yan et al., 2023
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Figure 3

Isl2a mutants show reduced thyroid function. (A) The assessment of tg expression in isl2a mutants at 3 dpf and 5 dpf via whole-mount in situ hybridization. At 5 dpf, isl2a-/- zebrafish had lower expression of tg and tshba as denoted by black arrows and arrowhead, respectively. Scale bar = 50 µm. (B) qRT-PCR analysis of the total expression of tg, slc5a5, tpo, tshba, and cga in isl2a+/+ and isl2a-/- larvae at 5 dpf. Error bars represent ± SD (n = 5, 15 fish per tube). (C) Both EGFP (green) and EdU immunoreactivity (red) were present in the thyroid of 120 hpf Tg (tg:egfp) wild-type (n = 7) larvae, whereas nearly no EdU-positive cells were detected within the tg domain in isl2a-/- larvae (n = 6). There were also no TUNEL-positive cells (red) in the tg domain at 127 hpf. Scale bar = 500 µm. (D) Thyroxine (T4) and 3,5,3′-triiodothyronine (T3) levels in the zebrafish body at 44 dpf. Error bars represent ± SD (n = 4). Asterisks indicate significant differences between isl2a+/+ and isl2a-/- groups (*P < 0.05, **P < 0.01).

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Endocrinol (Lausanne)