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Figure 2
Expression pattern analysis of capn3b in wild-type and capn3b mutant zebrafish embryos. (A) Whole mount in situ hybridization with the capn3b probe, not including the deleted regions, was performed in wild-type, capn3b mut1/mut1, capn3b mut73/mut73, and capn3b rnaless/rnaless embryos at 28 and 52 hpf. (B) Whole mount in situ hybridization analysis with the capn3b and unc45b probes in wild-type and capn3b rnaless/rnaless embryos at 72 hpf. For each sample type, at least 40 embryos were stained. Representative images are shown in the figure (hpf = hours post-fertilization).
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