Fig. 7

Histone H2A nuclear/cytoplasmic trafficking is essential for the negative regulation of histone H2A. (A) Effect of the inhibition of histone H2A nuclear/cytoplasmic trafficking on the protein degradation of cytoplasmic TBK1. (B) Effect of the inhibition of histone H2A nuclear/cytoplasmic trafficking on the protein degradation of nuclear TBK1. (C) Effect of the inhibition of histone H2A nuclear/cytoplasmic trafficking on the protein degradation of cytoplasmic IRF3. (D) Effect of the inhibition of histone H2A nuclear/cytoplasmic trafficking on the protein degradation of nuclear IRF3. For A–D), EPC cells seeded in 6-well plates were transfected with various indicated plasmids. After 36 h post-transfection, the cells were treated with LMB at the indicated concentration or left untreated. After 12 h, the cells were harvested and used for preparation of nuclear and cytoplasmic extracts. The expression ratio for TBK1 or IRF3 protein was quantified by Quantity One. (E) Effect of the inhibition of histone H2A nuclear/cytoplasmic trafficking on the histone H2A-mediated SVCV replication. (F) Effect of the inhibition of histone H2A nuclear/cytoplasmic trafficking on the expression of SVCV-N mediated by histone H2A. (G) Effect of the inhibition of histone H2A nuclear/cytoplasmic trafficking on the expression of SVCV-P mediated by histone H2A. (H) Effect of the inhibition of histone H2A nuclear/cytoplasmic trafficking on the expression of SVCV-G mediated by histone H2A. For (E–H), EPC cells seeded in 24-well plates were transfected with p3×FLAG or H2A-FLAG, then treated with LMB or left untreated. After 48 h posttransfection, the cells were infected with SVCV. Another 24 h later, the supernatants of infected cells were collected for the determination of virus titers. The cell pellets of infected cells were collected for qRT-PCR. Data represented means ± SEM (n = 3), and were tested for statistical significance. **p < 0.01; ns, not significant. The asterisk above the error bars indicates statistical significance using the group microinjected with empty plasmid as the control group. The asterisk above the bracket indicates statistical significance between the two groups connected by the bracket.