Fig. 1

Fig. 1

Fig. 1. Itga3b is expressed in the plasma membrane of cardiomyocytes. (A) itga3b is expressed in the ventricle and atrium of embryos at 48 and 60 hpf. Images of single confocal Z-stack are shown. (B) Membrane localization of Itga3b protein is observed in both the ventricle and atrium and the epidermis near the pericardium of wild-types (a) at 72 hpf. Low level of Itga3b protein is detected in the ventricle and atrium of itga3b morphants (b). Partial membrane localization of Itga3b protein is detected in the ventricle and atrium and the epidermis near the pericardium of itga3b^+/- mutants (c). No expression of Itga3b protein is observed in the ventricle and atrium of itga3b^−/− mutants (d). (C) Itga3b protein is expressed in the heart tube at 24 and 36 hpf and in the ventricle and atrium from 48 to 72 hpf, while a low level of Itga3b protein is observed in the ventricle and atrium at 96 hpf. Dorsal view (24 hpf) and ventral views (36–96 hpf) of embryos are shown. Respective 20 (a), 13 (c) or 5 (f) Z-stacks are shown. (D) Itga3b (red) is localized to the EGFP-expressing cardiomyocye plasma membrane of Tg(myl7:EGFP-Hsa. HRAS) transgenic embryos at 72 hpf. Itga3b protein (green) is observed in the cardiomyocyte plasma membrane surrounding nuclear dsRed (red) protein in Tg(myl7:dsRed) transgenic embryos. Itga3b (red) is not found in the EGFP-expressing endocardial cells of Tg(fli1:EGFP) or in the EGFP-expressing epicardial cells of TgBAC(tcf21:NLS-EGFP) transgenic embryos. Images of single confocal Z-stack are shown. a, atrium; e, epidermis; v, ventricle. Scale bar, 100 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)