Fig. 6.
akt1E18K mutant mesenchymal clones form premature pre-chondrogenic condensations. (A) In vivo confocal imaging of akt1E18K-GFP (green) mesenchymal clones at SL 3.7 mm reveals clonal expansion and premature pre-chondrogenic condensation in the caudal fin. Arrowhead marks the edge of condensation. (B,C) akt1E18K-GFP mesenchymal clones in the larval fin are hyperproliferative. (B) Quantification of mesenchymal cells expressing GFP or akt1E18K-GFP labeled with 5-ethynyl-2′-deoxyuridine (EdU) to assay DNA synthesis in the caudal fin. Data are represented as mean±s.e.m. n=number of analyzed fish. (C) Representative in vivo confocal images of EdU (magenta) labeling in the caudal fin of GFP (green) and akt1E18K-GFP (green) mosaic larvae at SL 3.5 mm. Arrowhead marks akt1E18K-GFP-expressing EdU-positive cells. Arrow marks EdU-positive wild type cells. SL, standard length in mm. Scale bars: 50 μm.