ZFIN Image: Wang et al., 2023, Figure 1

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Figure Caption

Figure 1

Generation of a zebrafish shank2b mutant using CRISPR/Cas9. (A) Structure of zebrafish shank2b gene and protein. The protein domains (ANK, ankyrin repeat domain; SH3, Src homology 3 domain; PDZ, PSD-95/Discs large/ZO-1 domain; SAM, sterile alpha motif domain) are aligned to the corresponding exons. Diagram of target site on 10th exon of zebrafish shank2b genomic DNA and a 14 bp deletion mutation by CRISPR/Cas9 gene editing. Predicted structures of wild-type (WT) and shank2b mutant proteins in zebrafish. The 14 bp deletion resulted in a stop codon on 13th exon and premature termination before the PDZ and SAM domains. (B) Reduced expression of shank2b mRNA in the brain of shank2b^−/− adult male zebrafish at 4.5 months post-fertilization (mpf) compared to WT fish, analyzed by RT-qPCR (WT n = 4, shank2b^−/− n = 4, **** p < 0.0001, Student’s t test). Data are shown as mean ± SEM. (C) The expression of shank2a mRNA in the brain of WT and shank2b^−/− adult (4.5 mpf) male zebrafish was not affected (WT n = 3, shank2b^−/− n = 4, ns, p = 0.1957, Student’s t test). Data are shown as mean ± SEM.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.