Figure 7

Depletion of pak2a in zebrafish induces neural tube defects, which were partially rescued by overexpression of WT‐PAK2, but not PAK2 mutations. A) The pak2a mutant pak2a ^Δ14, which contains a 14 bp deletion in the second exon, is generated using the CRISPR‐Cas9 system. B,C) An overt cavity in the third ventricle and diencephalon and a hemorrhage phenotype are observed in pak2a ^Δ14 mutants at 48 hpf. n = 20 for sibling, n = 31 for pak2a ^Δ14 mutants. D) The proportion of embryos with neural tube defects injected with mRNAs harboring human WT‐PAK2, PAK2‐P151S, PAK2‐E253A, and PAK2‐K278R mutants. n = 245 embryos for pak2a ^Δ14 without injection, n = 236 embryos for pak2a ^Δ14 with WT‐PAK2 injection, n = 95 embryos for pak2a ^Δ14 with PAK2‐P151S injection, n = 120 embryos for pak2a ^Δ14 with PAK2‐E253A injection, n = 232 embryos for pak2a ^Δ14 with PAK2‐K278R injection. Scale bar: 200 µm (B,C), 100 µm (D).