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Fig. 4
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Fig. 4
The Catecholaminergic system. A and B, Ventral views of whole-mount 6 dpf larval brains, anterior to the left, processed for dopamine (DA) immunostaining. Dopamine-immunoreactive neurons are present in the diencephalon and telencephalon with extensive descending and ascending fibre networks in the vmat2+/+ larval brain, but only scattered dopamine-immunoreactive neuronal cell bodies are visible in the vmat2−/− larval brain. Scale bar = 75 µm. C-E, Bar chart showing results of high-performance liquid chromatography (HPLC) assay on larvae of the indicated genotype at 6 dpf. Levels of dopamine (DA), noradrenaline (NA) and DOPAC. n = 6 for each genotype. F and G, Bar charts showing results of reverse transcription-quantitative PCR (RT-qPCR) assays on larvae of the indicated genotype at 6 dpf. Vmat2+/+ and vmat2−/− larvae presented similar levels of comta and mao transcripts. n= 5 for each genotype. H and I, Ventral views of whole-mount 6 dpf larval brains, anterior to the left, processed for tyrosine hydroxylase (Th1) immunostaining. Scale bar = 75 µm. J, Bar chart showing results of Th1-immunoreactive cells counting on larval brains of the indicated genotype at 6 dpf. n = 7 for each genotype. K and L, Ventral views of whole-mount 6 dpf larval brains, anterior to the left, processed for tyrosine hydroxylase (th1) RNA in situ hybridisation (ISH). Diencephalic neuronal clusters express th1 in vmat2+/+ and vmat2−/− larval brains. Scale bar = 75 µm. M, Bar charts showing results of reverse transcription-quantitative PCR (RT-qPCR) assay on larvae of the indicated genotype at 6 dpf. Vmat2−/− larvae exhibit increased levels of th1 transcript when compared to their vmat2+/+ siblings. n= 5 for each genotype. N and O, Ventral views of whole-mount 6 dpf larval brains, anterior to the left, processed for tyrosine hydroxylase 2 (th2) RNA in situ hybridisation (ISH). Scale bar = 75 µm. P, Bar charts showing results of reverse transcription-quantitative PCR (RT-qPCR) assay on larvae of the indicated genotype at 6 dpf. Vmat2−/− larvae exhibit increased levels of th2 transcript when compared to their vmat2+/+ siblings. n= 5 for each genotype. Data are mean ± SEM. Student's t test was used for statistical analysis. *P < .05. †P < .01. ‡P < .001
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Full text @ Acta Physiol. (Oxf).