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Figure 3
Expression levels of slc30 genes from zebrafish infected with P. aeruginosa. RT-PCR was performed on mRNA extracted from infected zebrafish at 6, 24, and 48 hpi. The dotted line at Y = 1 is the expression level of the genes in uninfected animals (control). Data are means ± SD of three independent experiments, and statistical analyses were carried out by two-way ANOVA and Tukey’s multiple comparison test. Asterisks show statistical differences versus uninfected zebrafish (**** p < 0.0001; *** p < 0.0005; * p < 0.05), and hash signs show statistical differences between 24 hpi and 48 hpi for slc30a4, and between 6 hpi and 48 hpi for slc30a7 (# p < 0.05).