Fig 7

Fig 7

A) Percentage of surviving and normally developing uninjected zebrafish larvae, larvae injected with 4 ng of RC morpholino, and larvae injected with different doses (1 ng, 2 ng or 4 ng) of crp3 SB morpholino. In the experiment, RC or crp3 SB morpholino was microinjected into the yolk sac of AB zebrafish embryos at the 1–4 cell stage and their survival and development was followed for 5 days. The percentage represents the live (black) and morphologically normal (gray) larvae in each group (n = 31–50) at 5 dpi. B) crp3 transcript levels in RC morpholino and crp3 SB morpholino injected AB zebrafish larvae at 0–5 dpi. In the experiment, 4 ng of RC or crp3 SB morpholino was microinjected into the yolk sac of AB zebrafish embryos at 1–4 cell stage and pools of 3–5 larvae (n = 7–8) were collected at specific time points. crp3 expression levels were measured with qPCR from technical duplicates and normalized with eef1a1l1 expression. A dot/circle represents the relative gene expression in a single pool of embryos/larvae, which was calculated using the 2^-ΔCt method, and the line depicts the median expression level. C) The survival of RC or crp3 SB morpholino injected homozygous (crp2^tpu6/tpu6) larvae after pneumococcal infection. In the experiment, 4 ng of RC or crp3 SB morpholino was microinjected into the yolk sac of crp2^tpu6/tpu6 embryos of F3 generation at 1–4 cell stage, and at 2 dpi the larvae were infected with 296 cfu of S. pneumoniae. The survival of infected larvae was monitored until 5 dpf. Data were collected from a single experiment, with the group sizes (n) indicated in the figure. The statistical comparisons of difference were calculated with Kruskal-Wallis test with Dunn’s multiple comparison in B, and with log-rank (Mantel-Cox) test in C. dpi = days post injection.